Which of the following methods is used to detect Senecavirus A?

The detection of Senecavirus A, which is known for causing vesicular disease in swine, is primarily accomplished through RT-PCR (reverse transcription polymerase chain reaction). This molecular technique is particularly effective for identifying viral RNA in samples, such as vesicular fluids, which are often collected from affected animals.

RT-PCR is sensitive and specific, allowing for the rapid detection of the virus even at low levels of viral RNA. This method works by converting the viral RNA into complementary DNA (cDNA), which can then be amplified and detected, confirming the presence of the virus.

Histopathology, serological assays, and whole genome sequencing can provide important information regarding Senecavirus A but are not the primary methods for direct detection of the virus. Histopathology involves examining tissue samples for lesions, which can suggest an infection but does not directly identify the virus. Serological assays detect antibodies against the virus, which indicates exposure or an immune response rather than the presence of the virus itself. Whole genome sequencing is used to analyze the genetic material of the virus in more detail but is not a front-line detection method in clinical settings.

Therefore, RT-PCR of vesicular fluids stands out as the most direct and reliable method for detecting Senecavirus A,