Which test is appropriate for diagnosing Senecavirus A?

The appropriate test for diagnosing Senecavirus A is RT PCR of oral, vesicular fluids. This method is highly sensitive and specific for detecting the viral RNA present in samples from infected animals, particularly those presenting with vesicular diseases. RT PCR allows for the rapid identification of viral genetic material, making it a valuable tool in both clinical diagnostics and surveillance.

Other diagnostic methods have limitations in the context of Senecavirus A. For instance, culturing the pathogen in eggs is less applicable since Senecavirus A primarily affects swine and does not culture well in avian systems, thus resulting in unreliable outcomes. A serological survey may be useful for epidemiological studies but does not confirm active infection, as it detects antibodies that may not indicate current disease. Microscopic evaluation of lesions provides valuable morphological information but cannot definitively identify the virus itself, as similar lesions can arise from different viral agents.